Figure 2
From: NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface
IL-10 rescues pregnancy failure upon NK cell depletion in FLT3-treated dams modulating decidual DC phenotype. (A) Representative flow cytometric histograms showing I-Ab and CD86 staining in gated decidual CD11c+ cells exposed to 1 μg/ml LPS in the presence or absence of IL-10 (10 ng/ml) or IL-10R (10 μg/ml). (B) IL-10−/− CD11c+ cells were co-cultured with IL-10+/+ NK1.1+ cells and exposed to 1 μg/ml LPS in the presence or absence of IL-10R (10 μg/ml), and flow cytometric histograms are shown, which are representative of four mice. (C) Summary data showing the percentages of I-Ab and CD86 in gated decidual CD11c+ cells compared to control cultures with or without LPS, IL-10 or IL-10R. Data shown are mean derived from seven mice per treatment. (D) Summary data showing the percentage of I-Ab and CD86 in gated decidual IL-10−/− CD11c+ cells compared co-cultures with IL-10+/+ NK1.1+ cells with or without IL-10R. Data shown are mean derived from four mice per treatment. (C,D) * and ** denote p < 0.05 and p < 0.01 respectively, as analysed by the Tukey’s test.
