Figure 6
Depletion of TCAB1 inhibits phosphorylation of ATR and its respective downstream target Chk1, resulting in DNA damage accumulation following EBV infection. (A,B) CNE1 and CNE1-LMP1 cells were treated with EBV, and some cells were further infected by shTCAB1 and shNC lentivirus for 24 h, followed by immunostaining for γH2AX. (C,D) Quantification of the results in (A,B) showing the percentage of nuclei containing >10 γH2AX foci among the 200 cells counted for each experiment. The statistical analysis was performed using Student’s t test (***P < 0.001). (E) CNE1 infected with EBV was subjected to western blotting using different antibodies. (F) After exposure to EBV, depletion of TCAB1 interrupted ATR-to-Chk1 signalling in CNE1 cells.
