Figure 1 | Scientific Reports

Figure 1

From: miR-100-5p inhibition induces apoptosis in dormant prostate cancer cells and prevents the emergence of castration-resistant prostate cancer

Figure 1

PDX models recapitulating PCa disease progression. PDX models recapitulating clinical course of therapy: (A) Two hormone-sensitive prostate PDX models were generated and subjected to surgical castration (androgen deprivation). Upon the emergence of a castration-resistant sub-line, pathological examination stratified the tumors into either adenocarcinoma (CRPC) or neuroendocrine (NEPC) phenotypes. Drawings are adapted from BioDigital Human Application (human.biodigital.com) who hold the copyright and permit this publication under an Open Access license. (B) Characterization of the fate of PDX models (abbreviations: LTL = living tumor laboratory, ADC = adenocarcinoma, Y = yes, N = no, UND = undetermined, CRPC = castration-resistant prostate cancer, NEPC = neuroendocrine prostate cancer). Evidence of therapy induced dormancy and resistance. (C) Surgical castration can induce tumor dormancy in vivo using PCa PDX models: (A) Immunohistochemical staining of tissues obtained from LTL-313B PDX model. Cell proliferation and apoptosis are detected with Ki67 and Caspase-3 antibodies measuring protein expression, respectively. (D) Quantification of proliferation and apoptosis in LTL-313B tumors through determining the number of positively stained cells/1000 cells in the pre-castration, dormancy and relapse phases.

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