Figure 4

Decreasing the front-to-rear difference of myosin II activity induced the collapse of lead front and reversal of soma translocation of Schwann cell-like OECs. (A–C) Images of migrating Schwann cell-like OECs before and after the frontal application of a gradient of DMSO as a control (A), latrunculin A (LA, 50 μM in micropipette, B) or ML-7 (5 mM in micropipette, C). (D–E) Summary of the percentages of collapsed or reversed cells in total observed cells (D) and migration rates of the soma (E) under various conditions. White arrowheads indicated the direction of the micropipette. Time, min; scale bars, 20 μm. Data were mean ± SD. ^** P < 0.01, compared with control, Student’s t-test.