Figure 7
Myosin II as a downstream signaling of repulsive factor Slit-2 mediated the reversal of soma translocation of OECs. (A–B) Immunocytochemical analysis of the distribution of p-MLC (green) with (B) or without (A) Slit-2 treatment. Dil dye (red) is a membrane marker. (C) Quantitative analysis of relative p-MLC intensity (normalized to control) at leading front of OECs with or without Slit-2 incubation. (D–E) Images of migrating OECs before and after exposure to a Slit-2 gradient with (D) or without (E) blebbistatin incubation. White arrowheads indicated the direction of micropipette. (F–G) Summary of the percentages of collapsed or reversed cells in total observed OECs (F) and average rate migration ratios of soma (G) in response to Slit-2 gradient under various conditions. Data were mean ± SD. ** P < 0.01, compared with control, Student’s t-test. Scale bars, 20 μm.
