Figure 2

PSMP protein could specifically chemo-attracted the CCR2⁺Ly6C^hi monocytes into the abdominal cavity with CCR2 dependent manner. (A) Ly6C^hi monocytes were sorted from wild type mice’s bone marrow, infused into CCR2 KO mouse (1 × 10⁷ cells per mouse) by i.v. and PSMP protein was injected into the abdominal cavity at the same time. (B) and (C) Forty-eight hours after the CCR2⁺Ly6C^hi monocytes from wild type mouse bone marrow were intravenously introduced into the circulation of the CCR2 KO mice, and the percentages of monocytes (CCR2⁺Ly6C^hi) in the abdominal cavities with and without PSMP were detected and statistically analyzed by flow cytometry. (D) and (E)The percentages of macrophage (CD11b⁺F4/80⁺) in the abdominal cavities of the adopted transfer mice with and without PSMP were analyzed by flow cytometry. Data were representative of at least two independent experiments with four mice per group. *0.01 < p < 0.05, **0.001 < p < 0.01, and ***p < 0.001.