Figure 4

PSMP expression in situ in the colon chemo-attracted Ly6C^hiCCR2⁺ monocytes in a CCR2-dependent manner. (A) After the wild type or CCR2 KO mice colons were infected with Ad-Null or Ad-PSMP in situ for 72 h, respectively, the immune cells that had infiltrated the lamina propria of the Ad-Null group, Ad-PSMP group and RS504393-treated Ad-PSMP group were detected by flow cytometry; And RS504393 was administrated by i.p. at the indicated time. (B) The immune cells infiltrated into the lamina propria of the five groups were analyzed by flow cytometry. (C) The main subset of the infiltrated immune cells in the Ad-PSMP wild type mice was analyzed by flow cytometry with the antibodies for CD45, CD11b, CCR2 and Ly6C, and the percentages of the CD11b⁺CCR2⁺ cells and CD11b⁺CCR2⁺Ly6C^hi cells among the total immune cells (CD45⁺) were quantified. (D) and (E) The total immune cell (CD45⁺) and monocyte (CD11b⁺CCR2⁺Ly6C^hi) infiltrations in the lamina propria of the five groups were statistically analyzed. Data were representative of at least three independent experiments with five mice per group. *0.01 < p < 0.05, **0.001 < p < 0.01, and ***p < 0.001.