Figure 5
From: The PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis
The neutralizing antibody of PSMP mollified DSS-induced colitis via its effects on the monocytes/macrophages and IL-6, TNF-α, CCL2, IFN-γ. Based on the DSS-induced colitis, PSMP neutralizing antibody 3D5 (5 mg/kg) or mouse immunoglobulin G (mIgG) as a negative control were peritoneal injected every other day. (A) The colons were separated from the normal, DSS-induced colitis and 3D5-treated DSS-induced colitis groups. (B) and (C) The lengths of the colons and the body weight of the three groups were measured and statistically analyzed. (D) The colonic tissue biopsies from the three groups were stained with HE. Scale bar = 100 μm. (E) and (F) The colonic inflammatory score of the three groups were statistically analyzed based on the conditions of the colonic epithelial injuries and immune cell infiltration of cLP. (G) The immune cells that infiltrated the cLP in the three groups were separated and analyzed by flow cytometry. The Gate in the FSC-SSC graph displayed the distribution of immune cells. (H) The immune cell (CD45+) infiltrations in the three groups were statistically analyzed. (I) The percentages of macrophages (F4/80+) among all of the immune cells in the cLP of the three groups were statistically analyzed. (J–L) The myeloid-derived cells (CD11b+) and granulocytes (Ly6G+) in the total immune cells of the three groups’ cLP were displayed in the CD11b-SSC graph and were statistically analyzed. (M) The inflammatory cytokines IL-6, TNF-α, CCL2, IFN-γ, IL-10 and IL-12p70 were detected in the colonic tissue homogenates by CBA, and the concentrations of these cytokines in the colon tissues of the three groups were statistically analyzed. Data were representative of at least three independent experiments with five mice per group. *0.01 < p < 0.05, **0.001 < p < 0.01, and ***p < 0.001.
