Figure 1
From: Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo
GCaMP2 is expressed in astrocytes and interneurons (A) Astrocytic and neuronal localization of GCaMP2 protein in organotypic cortical slices as shown by colocalization with the astrocyte-specific GFAP and interneuron-specific GAD-67 markers. Cells showing colocalization are marked by arrows. Scale bars: 50 μm. (B) Changes in GCaMP2 and GFAP expression as measured by average intensity within cell ROIs (n = 22–34 cells) in organotypic cortical slices as a function of days in culture (DIV). (C,D) Representative traces (top), I/V plot (middle) and action potential generating ability (bottom) of whole-cell patched GCaMP2 expressing astrocytes (C, n = 30) and neurons (D, n = 7) in organotypic cortical slices.
