Figure 1 | Scientific Reports

Figure 1

From: Hypoxia-inducible factor-1 alpha is involved in RIP-induced necroptosis caused by in vitro and in vivo ischemic brain injury

Figure 1

Determination of OGD and reoxygenation exposure times for HT-22 cells. (a and c) Digital hologram microscopy was used to acquire representative images showing changes in HT-22 cell number and morphology during the OGD and reoxygenation phases. Scale bar = 100 μm. (b) A growth curve was generated using absorbance values (490 nm) and cell viability was detected by MTS assay at 0, 4, 8, 12, and 16 h of OGD. (d) HT-22 cell absorbance values (490 nm) at 0, 3, 6, 9, and 12 h of reoxygenation were used to construct the growth curve. Doubling time during 0–3, 3–6, 6–9, and 9–12 h of reoxygenation was calculated. *p < 0.05, **p < 0.01 vs. control group. Also see Supplementary Figures S1, S2 and Videos S1, S2.

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