Figure 4

Overexpression analysis of the CsUGT75L12 gene in Arabidopsis thaliana. The function of CsUGT75L12 gene was verified by phenotype and metabolite variations in transgenic A. thaliana. (a) Phenotype of leaves and seeds of transgenic A. thaliana. (b) Expression level analysis of CsUGT75L12 gene in transgenic lines. (c) Identification and relative content of flavonol glycosides in the seeds of different lines. Each data point was present with the average mean of three reactions ± SD. Labelled columns not connected by the same letter are significantly different at P < 0.05, based on a Tukey’s honestly significant difference test.