Figure 4

Effect of EGCG on H2O2-induced changes in 67LR. (A) Immunocytochemistry analysis was performed using anti-67LR antibody and Alexa Flouor-488 conjugated secondary antibody(green). The nuclei were stained with DAPI(blue). VSMCs were pretreated with 10, 50, 100, or 150 μM EGCG for 2 h, followed by treatment with 200 μM H2O2 for 30 min. (B) Western blot showing the expression of 67LR after treatment with EGCG and H2O2. Results were expressed as Mean ± S.D. from 5 independent experiments. *P < 0.05 vs. H2O2 treatment group, #p < 0.05 vs. control group.