Figure 1

IgG removal of the uncrosslinked serum by various concentrations of PEG4000 (A) and PEG6000 (B). The resulting precipitate was analysed by SDS-PAGE with CBS, and immunoblotting with anti-IgG and anti-HSA antibodies. CBS-IgG, Coomassie blue stained IgG band; CBS-HSA, Coomassie blue stained HSA band; IB-IgG, immunoblotted IgG band; IB-HSA, immunoblotted HSA band.