Figure 4
From: Structure-based drug discovery for combating influenza virus by targeting the PA–PB1 interaction
Anti-influenza virus activity of PA-49. The anti-influenza virus effects of PA-49 were evaluated as described in the Methods section. MDCK cells grown in 24-well plates were infected and treated with serial dilutions of PA-49. At 48 h after infection, the cell morphology (a) was observed (scale bar; 150 μm) and (b) the antiviral effects on cells were measured by the WST-1 assay (closed circles) or CV staining (open circles). Optical density (OD) values (%) are expressed relative to the percentage of cells without virus infection. Viral titres in the supernatant (open triangles) were measured by TCID50 assays.
