Figure 1
From: Molecular hydrogen increases resilience to stress in mice
Repeated inhalation of hydrogen gas enhanced resilience to acute stress in mice. (A) Experimental procedure. After a 5-day adaptation period, the mice were given daily administration of saline, fluoxetine (10 mg/kg, i.p.), or inhaled mixture gas of H2/O2 [67%/33% (v/v)] or O2/N2 [33%/67% (v/v)] for 1, 3 h daily for 14 days. Beginning on day 15, behavioural tests were conducted to assess the depressive- and anxiety-like behaviours. Inhalation of hydrogen gas significantly decreased the immobility time of mice in the TST (B) and the floating time in the FST (C), increased the time spent in the central zone (D) without affecting the crossing activities (E) in the OFT, and decreased the latency to feeding (F) without affecting the total feeding in homecages (G) during the NSF test. *P < 0.05, **P < 0.01 versus the saline-treated control group. n = 8–11 per group. TST, tail suspension test; FST, forced swimming test; OFT, open field test; NSF, novelty suppressed feeding test.
