Figure 4 | Scientific Reports

Figure 4

From: MAP kinase-interacting serine/threonine kinase 2 promotes proliferation, metastasis, and predicts poor prognosis in non-small cell lung cancer

Figure 4

MNK2 actives AKT/4EBP1/eIF4E/MCL and ERK/eIF4E/MCL pathway. (a) A549, NCI-460 and NCI-1975 were treated with MEK inhibitor PD 0325901 and AKT inhibitor BEZ 235 (48h), respectively. The ERK and AKT downstream genes were detected by RT-PCR. Results were summarized as mean ± SD of 3 independent experiments (**P < 0.01). (b) Western bolt shown that inhibited MEK, both decrease MNK2 expression and eIF4E phosphorylation. Inhibited AKT, decreased MNK2 and eIF4E, 4EBP1 phosphorylation. (c) Silencing with siRNA-MNK2. RT-PCT of MNK2 response genes were down-regulated in the indicated cells. (d) Silencing MNK2 inactivated both 4EBP1 and eIF4E in the indicated cells. (e) p-eIF4E had a significantly positive correlation with MNK2 (**P < 0.01) in NSCLC tissues but not eIF4E. Representative IHC result was shown (original magnification: x40). Images presented in this panel were cropped from different parts of the same gel, or from different gels. The full-length gels are presented in the Supplementary Figs S2–S4.

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