Figure 5
LysEF-P10 rescued mice from lethal VREF infection. (A) Survival rates. Mice were injected intraperitoneally (i.p.) with 4 × 109 CFU of the VREF E. faecalis E028 strain. One hour later, various doses of LysEF-P10 were administered i.p. to treat the VREF-challenged mice (n = 10). As a control, mice were treated with an equivalent quantity of Tris-HCl buffer. (B) Colony counts. LysEF-P10 or an equivalent quantity of Tris-HCl buffer was administered i.p. to the mice at 1 h after the 4 × 109 CFU VREF E. faecalis E028 challenge. At the indicated times, the bacterial counts in the peripheral blood were determined. Black line: VREF-challenged mice treated with 5 µg LysEF-P10; red line: VREF-challenged mice treated with buffer; green line: mice pre-treated with 5 µg LysEF-P10, challenged with VREF, and treated with 5 µg LysEF-P10 1 h after challenge; blue line: mice thrice immunized with 50 µg LysEF-P10, challenged with VREF, and treated with 5 µg LysEF-P10 1 h after challenge. Values represent means ± SDs (n = 3).
