Figure 3 | Scientific Reports

Figure 3

From: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production

Figure 3

Overexpression of CYGB displays the anti-oxidant effect. (a) Representative images for DHE staining and quantitative analysis for hCPCs infected with CYGB- or vector-lentivirus for 48 hours. (b) Representative merged histogram and quantitative analysis for ROS measurement for hCPCs overexpressing CYGB vs. vector control by the FACS analysis with the ROS Detection Assay Kit (Deep Red Fluorescence). (c) Representative merged histogram and quantitative analysis for ROS measurement for hCPCs expressing shRNA against CYGB vs. scramble shRNA. The open blue traces are unstained cells as negative controls in panel b and c. (d) Examination of anti-oxidant gene expression at the mRNA level after CYGB was overexpressed. (e) Representative images and quantitative data of Western blot showing anti-oxidant protein expression level after CYGB was overexpressed. Full-length blots are presented in Supplemental Fig. 11. *Indicates p < 0.05 vs. control; **indicates p < 0.01 vs. control; n = 3 independent experiments.

Back to article page