Figure 1 | Scientific Reports

Figure 1

From: Mutation-Specific Mechanisms of Hyperactivation of Noonan Syndrome SOS Molecules Detected with Single-molecule Imaging in Living Cells

Figure 1

Single-molecule imaging of NS SOS dynamics. (a) Locations of NS mutations in SOS (M269, R552, and R1131) in the atomic structure (PDB ID: 3KSY15). Atomic structure of the G ___domain is unknown. (b) Expression of SOS proteins detected by immunoblotting analysis with anti-SOS1 antibody. (**Halo-tagged and *endogenous SOS). Full-length blot image is shown in Supplementary Fig. S2. (c) Typical micrographs of single SOS molecules on the cell surface before and after EGF stimulation for 8 min. The upper and lower micrographs show the same cells. Bar: 10 μm. (d,e) Translocation of SOS molecules to the cell surface. The molecules contained the REM(−) mutation (e) or did not (d). Densities of the fluorescent SOS molecules on the cell surfaces were normalized to the fluorescence intensity in the cytoplasm and plotted as values relative to that of WT SOS before EGF stimulation (time 0). Means of 6–10 cells are plotted with standard errors (SE). Asterisks indicate statistical significance relative to WT (d) or WT REM(−) (e) at the indicated times (p < 0.05 on Mann–Whitney test).

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