Figure 3
Increased abundance of tumor-infiltrating neutrophils in Trem1 +/+ mice. Colons from Trem1 +/+ and Trem1 −/− mice were analyzed by flow-cytometry either after the 3rd DSS cycle or at the final time-point. (a) Representative dot blots showing the relative frequency of live Ly6G+ neutrophils in CD11b+ cells and the “monocyte-waterfall”64 in live Ly6G− CD11b+ cells. (b) Relative frequencies of the indicated cell subsets among live CD45+ cells isolated from tumor-free colonic mucosa or tumors. (a,b) Analysis at the final time-point included n = 3 age-matched untreated control mice per group. For AOM/DSS treated mice at the final time point, tumor-free colonic mucosa and tumors were analyzed separately. In the Trem1 +/+ group, tumors were pooled to yield one tumor sample per mouse; in the Trem1 −/− group, tumors were pooled from n = 4 mice to yield a single tumor sample. Statistical testing was performed using 2way ANOVA.
