Figure 7
From: Human ATG3 binding to lipid bilayers: role of lipid geometry, and electric charge
Binding of ATG3 (but not GABARAP) to neutral NTs. (A) ATG3 but not GABARAP (labeled with Alexa 488) binds to PC:DOPE (70:30 mol%) NTs containing 0.05 mol% of Rho-PE as membrane marker. Scale bars: 20 µm. (B) Fluorescence intensities of Rho-PE (black) and ATG3-A488/GABARAP-A488 (red) from the pictures in A were analyzed using the Image J function “Plot Profile”. Here the x-axis represents the horizontal distance through the selection and the y-axis the vertically averaged pixel intensity. For each condition, at least 20 nanotubes from 3 independent experiments were analyzed. (C) From the confocal fluorescence images, the ratio of mean integrated fluorescence intensity of ATG3 and GABARAP-488 in membrane (IFmemb.) and solution (IFsol.) was obtained from radial profiles. In the box-chart and raw data (dots) representation, squares represent mean of each condition, the “whisker” represents minimum and maximum values. The box plot goes from upper (75) percentile, median and lower (25) percentile of the values. For each condition, at least 20 nanotubes from 3 independent experiments were analyzed. ***p < 0.001.
