Figure 4

Comparison of the medians of increased transcript levels of PP2A-C5 and AVP1 in six groups of transgenic plants. (a) The medians of PP2A-C5 transcript levels obtained from quantitative PCR analyses in six transgenic populations. (b) The medians of AVP1 transcript levels obtained from quantitative PCR analyses in six transgenic populations. Because the distribution of fold of change for PP2A-C5 or AVP1 transcript was not within the Normal distribution (p < 0.05), the median method and Wilcoxon rank test were applied to compare these six groups of transgenic plants. The transcript levels of PP2A-C5 and AVP1 relative to that of Actin2 in wild-type plant were set as 1. The fold of change for AVP1 or PP2A-C5 transcript was used to reflect the increased transcript levels of transgene transcripts in transgenic plants. The x-axis represents the fold of change for PP2A-C5 transcript (a) or AVP1 transcript (b). The transgenic plants containing corresponding vector were labeled on the left. An asterisk indicates a significant difference in comparisons between plants populations containing vectors A and D, B and E, and C and F, respectively. Statistical significance was determined by a Wilcoxon rank test at p < 0.05.