Figure 2

IL-8 does not prolong CLL cell survival in vitro. PBMC or monocyte-depleted PBMC (3 × 10⁶/ml) were incubated with or without recombinant IL-8 (20 ng/ml) for 10 days. IL-8 was added on days 0, 3, 6 and 9. Cell death of CD19⁺ lymphocytes was evaluated by flow cytometric alterations of light-scattering properties and confirmed by Annexin V staining on days 4, 7 and 10. Shown are mean ± SEM, n = 9. Statistical analysis was performed using Friedman test followed by the Dunn´s multiple comparison post-test. Asterisks indicate statistically significant differences (***p < 0.001) between PBMC and monocyte-depleted PBMC. The addition of IL-8 did not significantly modify cell survival of PBMC or monocyte-depleted PBMC.