Figure 10

RSS1 affects transcriptional regulation of PIF targets/shade responsive genes in Arabidopsis. Comparison of simulated shade (EODFR) mediated induction of transcript levels of selected shade responsive genes that are also well-known targets of PIF4/HBI1 such as (a) IAA19; (b) IAA20; (c) HFR1; (d) ATHB2; (e) EXP8 and (f) XTH15 in WT (Col-0), rss1 mutant and RSS1OE2 transgenic lines. The basal transcript levels in rss1, RSS1OE2 and pifq seedlings relative to the WT are given in inset. EODFR treatment caused more induction in the transcript levels of selected shade responsive genes in rss1 whereas the shade-mediated induction of these genes was highly reduced in RSS1OE2 line and pifq mutant seedlings as compared to the WT. Expression values represent the average of two biological replicates with three technical replicates each and error bars represent SE. (Student’s t-test; P < 0.05; *control vs. treatment; **WT vs. mutant).