Figure 2
From: Rational design of cholesterol oxidase for efficient bioresolution of cholestane skeleton substrates
Structural analysis of the PsChO-substrate complex. (A) Ribbon representation of the PsChO overall structure. The loop colored red stands is the flexible lid (L55-P77). FAD and the substrate dehydroepiandrosterone are shown as magenta and yellow stick, respectively. (B) Interaction in the cofactor-binding site between FAD (magenta) and PsChO (green sticks). (C) The predicted substrate binding model. The substrate analogue is shown as yellow stick. The orange sphere stands for active water molecular. (D) The oxygen channel proposed to function in access of dioxygen.
