Figure 6
Densitometry of immunflourescence staining of nitrotyrosine in left ventricle sections from sham (white bar) treated pigs or 72 h after multiple trauma and DCO (grey bar) or ETC (black bar) treatment. (B) Densitomery of immuneflourescence staining of caspase 3 in left ventricular tissue sections from sham (white bar) treated pigs or 72 h after multiple trauma and DCO (grey bar) or ETC (black bar) treatment. Representative PAS staining of left ventricular tissue after sham procedure (C) or after multiple trauma followed by DCO or ETC treatment. (D) Pixel density of PAS staining in left ventricular tissue sections from sham (white bar) treated pigs or 72 h after multiple trauma and DCO (grey bar) or ETC (black bar) treatment. (E) Pixel density of GLUT 4 staining in left ventricular tissue sections from sham (white bar) treated pigs or 72 h after multiple trauma and DCO (grey bar) or ETC (black bar) treatment. (F) Expression of GLUT 4 mRNA in left ventricular tissue assessed by qRT-PCR from sham (white bar) treated pigs or 72 h after multiple trauma and DCO (grey bar) or ETC (black bar) treatment, expressed as ratio of GLUT 4 mRNA to Gapdh mRNA. *p < 0.05, n = 6 each bar.
