Figure 7
From: Differences in the mechanical unfolding pathways of apo- and copper-bound azurins
Schematic showing the unfolding mechanism of apo- and holo-azurins from their native states (Napo and Nholo) to the unfolded states (Uapo and Uholo). The flux data through the parallel mechanical pathways was obtained through SMFS experiments and the structural information on the sequence of β-strand rupture and unravelling through SMD simulations. Apo-azurin follows both 2-state and 3-state pathways, whereas holo-azurin follows 2-state, 3-state and 4-state pathways. The intermediates on the unfolding pathways of apo- and holo-azurin are different.
