Figure 5

Gene expression analysis of macrophage markers in a small number of FACS-sorted macrophages. The plots represent relative expression of (A) Actb, (B) Arg1-M2 macrophage marker, and (C) Nos2-M1 macrophage marker in stored FACS-sorted splenic macrophages, stored FACS-sorted tumor macrophages, cultured murine BMDMs, and fresh FACS-sorted tumor macrophages by Microscale gene expression method. Three biological replicates were used for different macrophages. Each data point is represented as mean ± SEM from n = 3. The significant differences in mean Ct values of FACS sorted samples was compared to isolated murine macrophages by one-way ANOVA. ***Denotes p-value < 0.001, **Denotes p-value < 0.01, *Denotes p-value < 0.05 and ns stands for non-significant.