Figure 6
From: CXCL12/CXCR4-Mediated Procollagen Secretion Is Coupled To Cullin-RING Ubiquitin Ligase Activation
CXCL12 Up-Regulates Pro-Collagen Secretion. (A) Immunoblot of protein lysates (WCL) or conditioned media (MEDIA) from cells treated with vehicle, CXCL12 or TGFβ and probed for collagen 1 (COL1α1) or GAPDH (as WCL loading control) proteins. Ponceau staining is shown as loading control for conditioned media immunoblot. The WCL and MEDIA blots were loaded, run, and probed separately. (B,C) Graphs of densitometric analysis of replicate immunoblots of collagen 1 protein in whole cell lysate (WCL) (B) or conditioned media (MEDIA) (C). Collagen 1 protein is significantly downregulated in WCL from CXCL12-treated cells (#) but is significantly up-regulated in MEDIA from CXCL12-treated cells compared to vehicle (**p < 0.001; # or *p < 0.05; all value shown are normalized to vehicle protein levels). Graphs represent averaged measures of triplicate experiments.
