Figure 2 | Scientific Reports

Figure 2

From: RNA-Seq identifies genes whose proteins are transformative in the differentiation of cytotrophoblast to syncytiotrophoblast, in human primary villous and BeWo trophoblasts

Figure 2

(a) Immunoblot analysis of hCGβ expression in media (M) and lysates (L) of BeWo cells treated with forskolin (+) or vehicle control (−) as described in Materials and Methods. The last column shows the total (media + lysate) increase in hCGβ expression compared to vehicle-treated control. (Column values are adjusted based on sample volumes analyzed). (b) Immunoblot analysis of hCGβ expression in BeWo cells treated with forskolin (+) or vehicle control (−) as well as human villous placental extract (hPL) with (T) and without (U) deglycosylation treatment. In both samples, deglycosylation produced the 18 kDa band predicted by the amino acid sequence of hCGβ.

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