Figure 3 | Scientific Reports

Figure 3

From: A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae

Figure 3

LamB is a functional amylase. (a) Amylase activity of ITPG-inducible (+), GST-LamB fusions and catalytic site mutants, expressed in E. coli, was assessed by colormetric assay of cleavage of an artificial compound. Data are representative of three independent experiments represented by mean amylase activity, of triplicate sample, as measured by cleavage of ethylidene-pNP-G7 into p-nitrophenol, shown as mean amylase activity ± SD, n = 3 independent cultures.

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