Figure 1
From: The guanine nucleotide exchange factor Arhgef7/βPix promotes axon formation upstream of TC10
Expression of Arhgef7 in neurons. (a) A schematic representation of the Arhgef7 ___domain structure and the major isoforms is shown. DH: DBL homology ___domain, PH: plekstrin homology ___domain, CH: calponin homology ___domain, SH3: Src homology 3 ___domain, GBD: Git binding ___domain, CC: Coiled coil ___domain. (b) The expression of Arhgef7 in the cortex of E17 mouse embryos and postnatal mice (P0 or P2) was analyzed by Western blot (WB) using an anti-Arhgef7 antibody. The loading of comparable amounts of proteins was verified using and anti-actin antibody. Molecular weights are indicated in kDa. (c) Hippocampal neurons from E18 rat embryos were analyzed at 24 h (stage 2) or 72 h (stage 3) of culture by staining with an anti-Arhgef7 (green), the Tuji 1 or Tau-1 (red) antibodies and CellTracker Blue CMAC (blue) as a volume marker. The scale bars are 20 μm and 2 (upper panel) or 5 μm (lower panels), respectively.
