Figure 4
The injection of TNFα in hippocampus abolished the WTD-mediated modulation of hippocampal microglia, rescue of hippocampal neurons and the co-curation for NP behaviors. (A) The activation of microglia on the right side was quantified in CA1 and CA3 by both the area of cell body and the number of cells double stained by TMEM119 and TNFα (**P < 0.01, ***P < 0.001, Mean ± SEM, n = 3 mice/group), scale bar 50 μm. (B) The morphological alternations of neurons in CA1 and CA3 were analyzed by Golgi staining (n = 4 mice per group, right side). The figures on the left show the imaging of the entire neurons in Sham/SNL/WTD/WTD- TNFα groups, scale bar 100 μm; the scatter diagrams on the right show the statistical data of the intersections and total length of dendrites on both the apical and basal sides. (*P < 0.05, ***P < 0.001 present the significant differences between the Sham and SNL groups; $$$P < 0.001 present the significant differences between the Sham and WTD groups; ^P < 0.05, ^P < 0.001 present the significant differences between the Sham and WTD- TNFα groups; #P < 0.05, ###P < 0.001 present the significant differences between the SNL and WTD groups; &P < 0.05, &&&P < 0.001 present the significant differences between the WTD and WTD- TNFα groups; (Data are shown as Mean ± SEM, n = 4 mice/group). (C) The co-curation by WTD was abolished by PGB. Grouped data (Mean ± SEM, n = 8–10 mice/group) show the alternations in pain, depression and anxiety behaviors. In which, *** denotes the significant (P < 0.001) difference between Sham and SNL. ### denotes the significant (P < 0.001) difference between SNL and WTD. &&&denotes the significant (P < 0.001) difference between WTD and WTD- TNFα. ^^^ denotes the significant (P < 0.001) difference between WTD and WTD- TNFα. @@ P < 0.005, @@@ P < 0.001 denotes both the significant difference between WTD- TNFα and Sham.
