Figure 6
Migration of TE-derived cells in forebrain slices. (A) Bright-field images of E16.5 forebrain slices. DiI crystals were inserted within the putative migratory pathway of CalR-positive cells (arrows). (B) After incubation for 24 hrs, many DiI-labeled cells (arrowheads) were observed dorsal to the DiI application site (asterisk). (C,D) Immunostaining for CalR in vibratome sections of DiI-labeled slices. DiI-labeled cells were observed in the putative migratory pathway of CalR-positive cells (arrowheads in C). Many of them had a dorsally extended leading process (arrowheads in D). Yellow arrows indicate cell bodies. (E) Schematic diagram of slice culture. (F) Sagittal forebrain slices electroporated with GFP plasmid in the TE. (G,H) Time-lapse imaging of GFP-positive cells in the septal region. Arrowheads indicate dorsally migrating GFP-positive cells. ac, anterior commissure; di, diencephalon; sep, septal nuclei; 3 V, third ventricle. Scale bars: 25 µm in D, and 50 µm in G,H, 200 µm in B,C, and 1 mm in A,F.
