Figure 2 | Scientific Reports

Figure 2

From: Peroxisome Proliferator-Activated Receptor gamma negatively regulates liver regeneration after partial hepatectomy via the HGF/c-Met/ERK1/2 pathways

Figure 2

Ligand activation of PPARγ inhibits mouse liver regeneration after PH. (A) Liver to body weight ratio in untreated mice, rosiglitazone-treated mice, and GW9662-treated mice at different time points after PH. (B) Quantification of Ki67-positive hepatocytes and (C) PH3-positive hepatocytes. (D) Micrographs of liver sections immunostained with Ki67 & PH3 antibody from untreated, rosiglitazone- and GW9662-treated mice after PH. Scale bar: 200 μm. (E) Assessment of Cyclin D1 protein expression in the regenerating liver. Left panel: Western blot analysis of Cyclin D1 in untreated mice vs. rosiglitazone-treated mice. Right panel: Untreated mice vs. GW9662-treated mice. (F) Quantification of Western blot analysis for Cyclin D1 expression after analysis of three biological triplicates. (G) Real-time PCR analysis of Cyclin D1 mRNA expression after PH in untreated, rosiglitazone-treated, and GW9662-treated groups. *p < 0.05.

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