Figure 5
Calcipotriol inhibits clonogenicity of RDEB SCC cells. (a) RDEB-SCC cells were plated in T175 flasks (3 cells/cm2) and grown in the presence of 100 nM calcipotriol, 500 ng/ml LL-37 peptide or vehicle controls up to 18 days. Colonies were stained with crystal violet. Calcipotriol treatment significantly reduced colony number (b) and colony size (c + d), whereas LL-37 peptide showed no effect. Mean ± SD of three experiments is shown. Scale bar: 1 mm. (e) RDEB-SCC cells were cultured in the presence of 1–1000 nM calcipotriol or DMSO and viability assayed by MTT after 48 and 72 hrs. Data are presented as percentage of viable cells, relative to the vehicle treated control cells (100%, dotted line). Mean ± SD of at least 2 experiments performed in quadruplicates is shown. Statistical significance was calculated as described in the materials and methods section.
