Figure 8

PFEE-C and PFEE-W inhibited H22 cell migration in vitro. (a) After PFEE-C and PFEE-W treatment for 24 h and 48 h, H22 cell migration was observed by inverted microscope and analyzed by Image J. The width of scratches was shown in lower panels. (b) After PFEE-C and PFEE-W treatment for 24 h, proteins were isolated from H22 cells to detect the levels of MMP-2 and MMP-9 by Western blot. Cropped blots are shown and full-length blots are included in the Supplementary Information. The ratios of MMP-2/β-actin and MMP-9/β-actin were shown in lower panels. Data were analyzed by ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001 compared to untreated group.