Figure 3

In vivo observation of Paneth cell degranulation in SPF mice using moxifloxacin-based TPM. (a) Longitudinal moxifloxacin-based TPM images of intestinal crypts at the base after intraluminal injection of CpG-ODNs (top panel, 10 µM), and PBS (lower panel) at different time points. Longitudinal TPM images are presented at single representative depth planes in 10 min, 30 min, and 60 min post-injection. (b) A schematic of the small intestine showing the depth range of 3D TPM (red-arrow), and single representative depth plane (black-arrow). (c) Quantitative analysis of total granule pixel counts in the moxifloxacin-based 3D TPM images of degranulated intestinal crypts in SPF mice in 5 hours after intraperitoneal injection of CpG-ODNs. Statistical analysis was performed by Welch’s t-test; ****p < 0.0001. In this experiment, the number of mice used are as follows; wild type C57BL/6 mice bred in a SPF condition (n = 5). Scale bars in (a) indicate 100 µm.