Figure 2

SHP deficiency in liver parenchymal cells is associated with increased liver damage during ConA-induced hepatitis. (a) Chimeric mice were challenged with 30 mg/kg of ConA, and plasma ALT and AST levels were measured after 9 h (n = 3–7 per group). (b-g) Chimeric mice livers were collected 24 h after 20 mg/kg of ConA injection (n = 3–8 per group). (b and c) Representative either (b) H&E or (c) TUNEL staining images are shown. Black dotted lines in highlight necrotic area and TUNEL-positive cells are colored brown. Scale bar, 200 μm. (d-g) The bar graph represents mRNA expression levels of Tnfα, Il-1β, Il-6, or Il-10. Relative gene expression levels were normalized against the expression of 18 S rRNA. Data are means ± SEMs. *P < 0.05 (Tukey-Kramer HSD test after the one-way ANOVA).