Figure 4
Clinical sample validation. (a) PCA analysis was performed on CE-RAPD profiles of 210 K. pneumoniae samples from two hospitals using combination of three primers: P1283, P1254 and OPA-02. Pink indicate samples from NTUH, and green represent samples from TPECH. (b) The accuracy of the TIS system was assessed using a target pattern generated using NTUH_003. The target thresholds used for each primer are as follows: P1283: 0.0048, P1254: 0.0059 and OPA-02: 0.0173. Red mark represented the TIS of test sample was matched with target. PPV of TIS system was 100%. (c) The accuracy of the TIS system was assessed using a target pattern generated using NTUH_029. The target thresholds used for each primer are as follows: P1283: 0.0063, P1254: 0.0193, OPA-02: 0.0326 and CHL-07: 0.0092. Arrows indicate samples that were selected for further methods comparison analysis. Red arrows indicates target matched clusters as analyzed for three primers. Green arrows indicates target mismatched clusters. PPV of TIS system were 40% (three primers) and 100% (four primers). (d) Visualization of CE-RAPD profile similarity and identification of target cluster after addition of the extra primer, CHL-07. Samples were NTUH_029 matched cluster from three primer analysis. The TIS of samples are shown in brackets. Red font indicates match with the target, NTUH_029.
