Figure 2

Senescent phenotypes and cell cycle arrest in PRMT1-KD SK-N-SH cells. (A) Doubling-time and growth curves of control and PRMT1-KD SK-N-SH cells. Cell growth curves of control, PRMT1-A1-KD and PRMT1-B1-KD cells determined by direct cell counts are shown. Data are the mean ± standard deviation (SD) of triplicates. (B) Cell morphology of non-infected, control vector-infected, PRMT1 A1 or B1 shRNA-infected SK-N-SH cells. (C) Flow cytometry analyses of control or PRMT1-KD SK-N-SH cells with A1 and B1 shRNA. All experiments were performed at least three times. The data are shown as the mean ± SD. ^* indicates p < 0.05 and ^** indicates p < 0.01 compared with non-infected SK-N-SH cells. (D) Non-infected, control vector-infected, PRMT1 A1 or B1 shRNA-infected SK-N-SH cells were fixed and stained for SA-β-Gal.