Figure 5

ROS production, sensitivity and signaling in PRMT1-KD SK-N-SH cells. (A) ROS production in non-infected, control vector-infected and PRMT1 A1 or B1 shRNA-infected SK-N-SH cells as shown by the produced DCF-DA. (B) RNA levels of p38 in non-infected, control vector-infected, and PRMT1-A1 or B1 shRNA-infected KD SK-N-SH cells determined by RT-qPCR. (C) Western blot of p38 in non-infected, control vector-infected, and PRMT1 A1 and B1 shRNA-infected SK-N-SH cells (100 μg of protein). (D) Survival of cells after addition of H₂O₂. Experiments were performed at least three times. The data are shown as the mean ± SD of triplicates. ^* indicates p < 0.05 and ^** indicates p < 0.01 compared with non-infected SK-N-SH cells.