Figure 6
Migration activity of SK-N-SH cells increased after PRMT1-KD through PAI-1 induction. (A) The pre-migration (0 h) and post-migration (16 h) images of control vector-infected, PRMT1 A1 or B1 shRNA-infected SK-N-SH cells are shown. The white circles indicate areas covered by the stoppers before cell migration. The area inside the white circle covered by cells is the migration area. Migration activity of the cells were quantified as the percentage of the migration area of the PRMT1 shRNA-infected cells compared to that of the control vector-uninfected SK-N-SH cells (**P < 0.01). (B) The cells that migrated to the bottom surface of the membrane after 22 h of Transwell migration assay were fixed and stained with crystal violet. Representative images of PRMT1 A1 shRNA-infected or control vector-infected SK-N-SH cells were shown. Five randomly selected fields were imaged and the number of the migrated cells was counted and data are shown as the mean ± SD. ** indicates p < 0.01 compared with non-infected SK-N-SH cells. (C) The pre-migration (0 h) and post-migration (16 h) images of PRMT1 B1 shRNA-infected SK-N-SH cells treated with a PAI-1 inhibitor PAI-039 at the concentration of 0, 10, and 50 μM. Migration activity were quantified as the percentage of the migration area of the treated cells over untreated cells (**P < 0.01). (D) Western blot analyses of PAI-1 in the spent media of non-infected and PRMT1-A1 shRNA-infected SK-N-SH cells. T indicates total lysate. CM24 and CM48 indicate serum free culture medium from 24 h and 48 h cultures concentrated by ultracentrifugation.
