Figure 7
The requirement of p53 to induce p21 and PAI-1 in non-MYCN-amplified neuroblastoma cells. (A) The SK-N-SH cells infected with PRMT1 A1 plus B1 shRNA at the fifth day were treated with a p53 inhibitor pifithrin-α (10 μM) or not for 48 h. The RNA levels of the p53 target p21 and PAI-1 of the non-infected and PRMT1-KD SK-N-SH cells treated with pifithrin-α or not were determined. (B) Western blot analyses of cell extracts (20 μg of protein) from control vector infected, PRMT1 A1 or B1 shRNA-infected SK-N-AS cells with anti-PRMT1 or ASYM24. Detection by anti-β-actin was used as a loading control. (C) RT-qPCR analyses of RNA levels of PRMT1, p53, p21 and PAI-1 in non-infected, control vector-infected, and PRMT1 A1 or B1 shRNA-infected SK-N-AS cells. Experiments were performed at least three times. The data are shown as the mean ± SD of triplicates. ** indicates p < 0.01.
