Figure 5 | Scientific Reports

Figure 5

From: Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9′-methyl-3′-carbazole)-3, 4-dihydro-β-carboline

Figure 5

MCDC inhibits cell cycle-related protein expression and AKT phosphorylation in MCF7 cells. (A) Heatmap and hierarchical cluster analysis of differentially expressed genes in MCF7 cells treated with MCDC. MCF7 cells were treated with MCDC (5 μM) for 24 h. Total RNA was extracted, and genomic profiling was examined by Illumina Human HT-12 v4 BeadChips. Criteria for selecting MCDC-responsive genes: fold change > 2.5X and P < 0.05. (B) Results of Ingenuity Pathway Analysis of MCDC-responsive genes. (C) Cell cycle analysis on MCF7 cells after MCDC treatment. MCF7 cells were stained with propidium iodide and subjected to flow cytometry analysis after 24 h of treatment with 6 μM MCDC. The stacked bar chart summarizes three independent cell cycle experiments. (D–F) Immunoblots of S phase-related proteins. The bar charts incorporate data from three independent western blots. (D) CDK1, (E) CDC25C, and (F) MIF downstream protein AKT and phospho-Ser473 AKT, all at 48 h after treatment. All data are presented as means ± SDs of three independent experiments. *P < 0.05; **P < 0.01 by Student’s t-test.

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