Figure 2

PI3K/Akt/mTOR pathway inhibitors mitigate the suppression of autophagy induced by LDL. (A) HUVECs were treated with LDL at the indicated concentrations for 60 min in the absence or presence of Rapamycin (Rap, 250 nM for 30 min). The expression of autophagy-associated and PI3K/Akt/mTOR pathway proteins is shown. (B) HUVECs were treated with PI3K inhibitor LY294002 (5 μM) for 30 min and/or LDL (50 μg/mL) for 60 min. The expression of autophagy-associated proteins and PI3K/Akt/mTOR pathway proteins was analyzed. The expression in Ctr group cells was assigned the value of 1, n = 3, ^*p < 0.05, ^**p < 0.01 versus Ctr, ^&&p < 0.01 versus LDL (10 μg/mL), ^ффp < 0.01 versus LDL (50 μg/mL), ^δp < 0.05 versus Rap, ^#p < 0.05, ^##p < 0.01 versus LDL. Data expressed as mean ± S.E.M.