Table 1 Substrate kinetic parameters.
From: Molecular architecture of the Jumonji C family histone demethylase KDM5B
Substrate peptide | H3(1–8)K4me3 | H3(1–10)K4me3 | H3(1–15)K4me3 | H3(1–21)K4me3 |
|---|---|---|---|---|
Kcat *104 (s−1) | 51.9 ± 2.5 | 57.9 ± 3.8 | 35.7 ± 1.7 | 29.1 ± 2.7 |
\({{{\rm{K}}}_{{\rm{m}}}}^{{\rm{app}}}\) (µM) | 4.2 ± 0.8 | 19.1 ± 3.8 | 1.0 ± 0.3 | 1.1 ± 0.5 |
95% Confidence Intervals | ||||
Kcat *104 (s−1) | 46.7 to 57.1 | 50.0 to 66.0 | 32.1 to 39.3 | 23.4 to 34.8 |
\({{{\rm{K}}}_{{\rm{m}}}}^{{\rm{app}}}\) (µM) | 2.6 to 5.8 | 11.2 to 27.0 | 0.4 to 1.6 | 0.2 to 2.1 |
R² | 0.9283 | 0.9251 | 0.8525 | 0.7098 |
\({{\rm{K}}}_{{\rm{cat}}}/{{{\rm{K}}}_{{\rm{m}}}}^{{\rm{app}}}\) *104 (s−1/µM) | 12.4 | 3.0 | 36.1 | 25.3 |
