Figure 8

Effects of RYP dry extract on protein expression. MCF-7 cell lines were incubated without or with RYP dry extract at 1 mg/ml, 3 mg/ml,10 mg/ml for 24 h. The proteins of cells were extracted and determined by western blotting. The pictures of BCl-2 (A), bax (A), parp (C) and p-Akt (D) were taken. Then protein pictures were analyzed with software. The BCl-2 (B), Bax (E), parp (G) and p-Akt (H) protein expressions were obtained and ratios of Bax to BCl-2 were calculated (F). Each experiment was done in triple.