Figure 1

Identification and characterization of lncASIR (A). Heatmap of RNA-sequencing from differentially expressed mRNAs in insulin treated primary mature adipocytes, Lep, Acss2 and Fasn marked for reference. (B) Heatmap of RNA-sequencing for differentially expressed lncRNAs from same dataset as 1A. LncASIR and lncbate1 is labelled on the side of heatmap for reference. (C) Validation of RNA-seq with Quantitative RT-PCR from insulin treated adipocytes for LncASIR, Fasn and Glut4. (D) Quantitative RT-PCR from tissue panel for LncASIR expression, RPL23 used as to calculate ΔΔCT. (E) Cellular fractionation from adipose tissue for localization of LncASIR (n = 3). 47S and Gapdh used as nuclear and cytosolic control respectively. (F) Expression of LncASIR during insulin treatment time course in mature adipocytes (n = 6). (G) Expression of LncASIR during adipocyte differentiation time course by Quantitative RT-PCR (n = 4). (H–J) Expression of LncASIR by Quantitative RT-PCR in 8 weeks old mice fat pads upon overnight fasting (n = 5). (K–M) LncASIR expression in high fat diet mice. Mice were fed HFD starting from 3 weeks old for 3 months (n > 6). Unpaired t-test was used to calculate p-value. *P < 0.05. Error bars are SEM. Graphs have been drawn using Prism 8.