Figure 3 | Scientific Reports

Figure 3

From: Silencing an insulin-induced lncRNA, LncASIR, impairs the transcriptional response to insulin signalling in adipocytes

Figure 3

Silencing of LncASIR using dcas9-KRAB lentiviral system in primary white adipocytes. (A) Quantitative RT-PCR data for silencing efficiency of LncASIR with and without insulin treatment. (B) Oil-Red O staining after knockdown. Representative well have been displayed. (n = 4) (C) Semi-quantitative measurement of Oil Red O (n = 4). (D) The Venn diagram of the downregulated genes caused by sgRNA.1 and sgRNA.2 in adipocytes at basal level. Threshold for the gene selection: logFC < −0.4 and p < 0.2 (E) Same as panel D with insulin treated adipocytes. (F,G) Pathway analysis for the gene list from panel D and E using DAVID, respectively. Unpaired t-test is used to calculate p-value. *P < 0.05; Error bars are SEM. Graphs have been drawn using Prism 8.

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